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    التشخيص المعملي للفيروسات

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    تاريخ التسجيل : 22/03/2010
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    التشخيص المعملي للفيروسات

    مُساهمة من طرف admin في الخميس يناير 10, 2013 11:13 pm

    Laboratory Diagnosis of Viruses

    Direct detection of the virus
    The diagnosis depend on detection of the virus itself i.e.virus antigen

    Infection Immunological response detection
    The diagnosis depend on immunlogical response for the body of the host

    Direct detection of the virus
    Electron microscopy
    Cytological Examination
    Virus Isolation
    Virus Antigen Detection


    Electon Microscopy
    Identification of the virus morphology in direct speciment or in viral culture
    for identification of HBV,VZV,HSV
    Sometimes we use Spacific Ab to accumulate the viral particle

    Cytological Examination
    To see the viral effect on cell culture,these effect might be:
    Cytopathic Effect(CPE) eg: in HSV,CMV
    Inclusion body formation within the cell eg:in CMV
    Syncytia i.e.cell clumping and fusing in the form of a giant cell eg: in VZV
    Nuclear Inclusion i.e formation of inclusion within the cell nuclear
    Nucleo-Cytoplasmic inclusion i.e inclusion within the cell nucleos and cytoplasm
    Cell lysis i.e. the cell itself lyse
    . Cell transformation eg: in HIV,HBV
    .Interferance with the cell function i.e. cells become liable to abnormal change eg:CPE
    Hemoadsorption I.e the cells agglutinate like in Influenza virs infection

    Virus Isolation
    We culture the virus in living cells
    Cell cultures line
    Chick Embryo
    Animal Innoculation

    Cell culture
    Cells and media in containers containing growth factors these,these factors could be serum,vitamines,electrolytes,etc
    cell culture classified according to it's continuity into:
    Primary cell culture
    It's a normal like epithelium,it live for short period then die as it can't divide forever to surviveeg: Human Fetal Lung cells
    Semicontinue cells line
    It has an ability to divide for relatively long period of time,but eventually will die,it contain a malignant caracteristic.
    Continuous cells line
    This is a malignant/cancereous cells,it has the ability to devide continuously,eg: HeLa,hep2,Vero
    Lymphocyte cell culture
    This may be T-cell or B-cell,it used for HIV,HTLV1&2,EBV
    ****l vial culture
    It has ability to detect the viruse through addition of Abs,eg: in CMV diagnosis

    Genetic engineering cell lines
    This one is genetically modified


    Chick Embryo

    This is an old technique depend on ejection of chick embryo with the virus,then studying the effect of the virus on the embryonic growth
    Amniotic innoculation
    In 10-11 old egg,eg: Infleunza virus effect on chick embryo lung
    Allantoic innoculation
    For vaccine development,collection of the fluid after 24 hours injection
    Chorioallantoic
    For Poxvirus,HSV study

    Animal Innoculation
    Ejection of the virus in animal,then see the effect of the virus on animal,it also used in the development of some kind of vaccines eg:Rabies virus in dog brains for development of antirabies vaccine.



    Virus Antigen Detection
    This one depend on detection some part of the virus,these parts are:
    Detection of Viral protien
    Viral Nuclear Acid Detection


    Detection of Viral Protien
    This one is done by the same methods like the other immunlogical response methods,so it will be discussed together with the other micrbiological tests.
    Viral Nuclear Acid Detection


      الوقت/التاريخ الآن هو الخميس ديسمبر 14, 2017 6:26 am